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CELLine - Disposable bioreactor for efficient protein expression
The cell culture flask CELLine is a disposable bioreactor based on two-compartment technology. It allows to cultivate cells at highest densities (10^7-10^8 cells/ml) and to express proteins at high concentrations.
The bioreactor CELLine is separated into a medium and a cell compartment by means of a 10 kDa semi-permeable membrane. This membrane allows a continuous diffusion of nutrients into the cell compartment with a concurrent removal of any inhibitory waste product. The individual accessibility of the compartments allows supplying cells with fresh medium without mechanically interfering with the culture. Optimal oxygen supply and control of carbon dioxide levels is ensured by a silicone membrane which forms the cell compartment base.
The bioreactor CELLine is suitable for both animal and plant cells either growing in suspension or as adherent cells. Two types are available, ideal for suspension cells, adherent cells in combination with microcarriers, e.g. hybridoma cells, or anchorage-dependent cells.
Special features:
- Easy operation - as simple as using a tissue culture flask
- Cost efficient - 90 % less media supplements
- Reduced handling time
- Efficient protein expression - 50x higher concentrations
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Bottles have reference graduations and are designed to be complient with System 45 adapters and accessories. Supplied with PBTP cap and PTFE faced silicone liner. Additional caps and liners are available (see Article Nos. 1098 and 8275)
Single neck with 32 or 45 threaded top. This design allows access to sample without a spatula or policeman. One thread size allows for use with a variety of apparatus including rotary evaporators. Flask may be easily stored with screw cap closures. Flask fits standard heating mantles.
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High quality borosilicate glass flasks have been specifically designed for freeze drying and feature a wide mouth opening for easy access to the dried material. Working volume: 125 mL. All Thermo flasks feature a 75mm diameter opening for facilitating the use of a single universal flask lid that is interchangeable between all different flask sizes.
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15x45mm Screw Thread Vials
- Compatible with Waters® WISP™ 48 position, 717, Breeze™ and Shimadzu® autosamplers
- Use for waste/wash vials for other autosamplers
- 13/425 open top caps and septa
- StepVial design precisely aligns insert and vial
- Economical kits simplify ordering
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Labware
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