The Eclipse™ 3+ represents Wyatt Technology's fourth generation AF4 system and is the market-leading separation system using the Asymmetric-Flow Field-Flow Fractionation (AF4 or FFF) technique. The Eclipse™ 3 integrates easily with Wyatt's Multi-Angle Light Scattering (MALS) instruments: DAWN®, miniDAWN™, as well as the Optilab™ rEX. The combined system provides absolute size and molar mass distributions of the particles/molecules separated by the AF4 unit. The combined system solves the most difficult sizing tasks in the macromolecular and colloidal arenas. Its resolution is similar to sedimentation equilibrium, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.