Batch bioreactor / fermentor RoboLector XT

laboratoryautomatedfor parallel synthesis

The RoboLector XT system is an automated fermentation platform which combines the high-throughput fermentation and online monitoring capability of the BioLector XT microbioreactor with the precise and accurate liquid handling of a robotic system. The RoboLector system autonomously prepares media compositions, among others, from the design of experiments (DoE) tests, followed by an online-monitored high-throughput cultivation. Furthermore, pH adjustments can be made and inducers as well as feed solutions can be added to each cultivation well according to a pre-defined schedule or triggered by online process signals. Automated sampling into various targets including a cooling station facilitates optimal process monitoring and control. Cultivation Modes • DO or time controlled feeding • Fed-batch with bolus feeding • Repeated fed-batch • Biomass dependent sampling or dosing • Time dependent sampling or dosing • pH adjustments Online Trigger Signals • Biomass concentration • pH, DO (using optodes) • Fluorescent molecules (GFP, YFP, DsRed ...) • NAD(P)H and riboflavins • Process or induction time • Working volume Advantages • Automated upstream processing of up to 48 parallel cultivations • Continuous operation 24 hours/day and 7 days/week • Plug & Play disposable technology • Design of experiments (DoE)• Detailed process understanding in short time • Excellent pipetting accuracy (< 5 %, 50 - 950 μl) and reproducibility (CV < 5 %, 50 - 950 μl) • Reliable scale-up to lab-fermenters • Fast and easy data analysis • A valuable tool for PAT and QbD • Processing units possible (for custom made solutions1) Applications • Automated sampling • Automated induction