MuVi SPIM CS Enabling 3D Imaging of Optically Cleared Samples
The sample chamber, the illumination, and the detection unit in the octagon are adapted to match the experimental needs in terms of sample size, refractive index, field-of-view, and resolution, adding high flexibility to the system.
Cleared Sample Module
The Cleared Sample Module makes the MuVi SPIM geometry suitable for large cleared sample imaging. The exchangeable cleared sample octagon and an overhead sample positioning unit installed on the microscope’s top plate makes it fully compatible with the live sample version of the MuVi SPIM.
The octagon can be easily removed from the microscope for maintenance and cleaning of the sample chamber and the objective lenses that have been exposed to the mounting solution. The sample chamber is compatible with a broad range of solutions used in different clearing protocols. Available in two different sizes (max. volumes of 20 ml and 33 ml), the sample chamber can accommodate large samples such as mouse brains and other complete organs. Sample mounting from above enables easy sample access and optimized travel range suited for various mounting methods (e.g. hook, plate, pin or cuvette).
Flexible illumination and detection configurations leverage the MuVi SPIM CS to meet your application needs and allow imaging of a great variety of samples at high speed and high resolution.
Illumination and Detection
The MuVi SPIM CS can achieve a resolution down to 300nm in 3D (at a wavelength of 500nm), enabling imaging of large optically cleared samples with high resolution.