Raman microscope TCS SP8 CARS

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Staining is unnecessary when imaging specimens with the TCS SP8 CARS (Coherent anti-Stokes Raman Scattering) confocal microscope. The CARS technology exploits image contrast arising from characteristic vibrational states of different molecules within a specimen. As no labeling is required, the specimen remains almost unaffected from preparation and imaging. Users benefit from its ability to image, at high speed and resolution, a wide range of live specimens, including cells, tissues, and even small organisms. To achieve this performance, the microscope offers visible, infrared (IR), and ultraviolet (UV) lasers, second harmonic generation (SHG), and CARS. Due to its flexibility and convenient handling, the TCS SP8 CARS is a superb instrument for research institutes and multi-user facilities. Key Features Imaging of native specimens without dyes The limitations of dye-based imaging methods are overcome via integration of the label-free CARS technology into the TCS SP8 confocal platform. No staining of the specimen is needed as image contrast is provided by the characteristic, intrinsic vibrational states of the different molecules in the specimen. Thus, CARS does not suffer from photobleaching and staining artifacts. Multispectral imaging with VIS, UV, IR, SHG, and CARS The TCS SP8 CARS platform provides various imaging methods, such as Coherent anti-Stokes Raman Scattering (CARS), single photon or multiphoton fluorescence, second harmonic generation (SHG), or imaging with infrared (IR), visible (VIS), and ultraviolet (UV) lasers in simultaneous or sequential mode.