Data workstation CellCelector CLD

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Overview
The CellCelector CLD is an automated cell imaging and isolation platform designed for cell line development (CLD) workflows. It integrates high-sensitivity brightfield and optional multi-channel fluorescence imaging with automated single-cell and colony picking to support high-throughput clone screening, monoclonality documentation and downstream expansion.

Why CellCelector CLD

• Accelerates clone screening and productivity assessment with high-speed scanning and intelligent imaging
• Detects low-expression targets with enhanced sensitivity and shading correction
• Provides audit-ready image and metadata documentation for regulatory traceability
• Enables high transfer efficiency and viability for reliable downstream expansion

How it works

• Seed candidate pools into Next Generation CLD Nanowell Plates to maximize single-cell occupancy and minimize cross-contamination
• Perform automated brightfield imaging and segmentation to identify single-cell nanowells and exclude multicell wells
• Track lineage and clonal expansion across timepoints to document monoclonality
• Use optional fluorescence channels to quantify secretion/binding and enable multiplexed productivity ranking
• Isolate and transfer top clones with a gentle single-cell picking arm preserving viability
• Automatically capture images and metadata (plate maps, timestamps, analysis masks, decision criteria) for audit-ready records

Key features and product highlights

• High-content imaging with dual objectives (4× for wide-field scanning, 10× for verification)
• Optional five-channel fluorescence unit for marker-based selection and quantitative ranking
• Next Generation CLD Nanowell Plates: screening up to 85,000 candidate clones per plate
• Single-use 80 µm glass capillary for gentle, high-precision nanoliter aspiration
• Heated deck tray to maintain stable 37°C conditions during operations
• Single curved frameless monitor station for consolidated image, metadata and table views
• Maintenance-free design to reduce routine downtime
• Automated documentation and traceable audit trails to support clonality evidence

Performance and claimed benefits

• Reported transfer efficiency and viability up to 100% for retrieved cells
• Screening throughput up to 85,000 candidate clones per Next Generation CLD Nanowell Plate
• Demonstrated ~40% more monoclonal wells and >3× higher clone outgrowth versus standard plates in comparative tests
• Rapid identification of top clones from large candidate pools for faster downstream development

Applications and target users

• Bioprocess and CLD teams scaling antibody and recombinant protein production
• Research and development labs prioritizing throughput, reproducibility and documented clonality
• Programs working with low-expression targets requiring sensitive imaging and multiplex screening

Example data and resources (descriptive)
Provided example workflows and screens include Day 4 productivity assays (brightfield, false-color and fluorescence channels), imaging and growth screenshots that illustrate monoclonality verification and pick-readiness metrics.

Technical specifications

• Model: CellCelector CLD (automated imaging and single-cell/colony picking platform)
• Optional fluorescence: up to five channels for marker-based selection and quantitative ranking
• Microscope objectives: 4× (wide-field scanning) and 10× (high-resolution verification)
• Picking capillary: 80 µm single-use glass capillary for nanoliter aspiration
• Deck temperature control: heated deck tray to maintain 37°C during operations
• Nanowell plate capacity: supports screening up to 85,000 candidate clones per plate
• Imaging enhancements: shading correction algorithm and advanced sensitivity for low-expression targets
• Transfer performance: up to 100% transfer efficiency and viability (reported)
• Operation: maintenance-free daily operation to reduce downtime
• Documentation: automated image and metadata capture (plate maps, timestamps, analysis masks, decision criteria)
• Demonstrated cell types: CHO (including CHO K1), HEK293, hybridoma; plate coatings available for ultra-low attachment and plasma coatings to support iPSC and cancer cell lines