Mass spectrometer timsTOF Pro 2

MSQTOFion mobility

Overview
timsTOF Pro 2 is a TIMS‑QTOF mass spectrometer combining Dual‑TIMS ion mobility with QTOF detection and PASEF® (parallel accumulation serial fragmentation) workflows. It is designed for high‑throughput 4D‑Multiomics applications including 4D‑Proteomics™, 4D‑Metabolomics™ and 4D‑Lipidomics™ where mobility, speed and identification confidence are required.

Highlights

• Speed – PASEF® delivers sequencing rates >120 Hz without compromising resolution.
• Depth – Ion mobility adds a fourth separation dimension, improving data completeness and peak capacity.
• Robustness – Instrument architecture supports routine operation across large sample series with minimal maintenance.

Key Features
Dual‑TIMS architecture enables parallel accumulation and sequential release to maximize ion capacity and duty cycle. Integrated ion optics and QTOF detection provide high sensitivity and mobility‑aligned MS/MS spectra. CCS (collisional cross section) measurement is supported to assist compound identification and library matching.

• Dual‑TIMS analyzer for increased ion capacity and near‑continuous duty cycle.
• PASEF® optimized for high‑speed MS/MS with mobility‑aligned precursor/fragment mapping.
• CCS‑enabled acquisition to improve identification confidence and reduce false positives.
• Supports dia‑PASEF® and prm‑PASEF® workflows for comprehensive and targeted proteomics.

Benefits
Mobility separation concentrates analyte ions and reduces interference from singly charged species, producing cleaner MS/MS spectra and improving sensitivity, selectivity and reproducibility for both discovery and targeted experiments.

• dia‑PASEF®: efficient combination of DIA and PASEF® for high coverage and confident identifications.
• prm‑PASEF®: high‑throughput targeted quantitation with hundreds to thousands of precursors per acquisition.

Performance Examples

• Sequencing speed >120 Hz in PASEF® mode.
• Nearly 100% duty cycle achievable with Dual‑TIMS.
• Approx. 3× higher ion capacity with Dual‑TIMS analyzer.
• Example: ~9,000 protein groups identified from 200 ng K562 lysate in 35‑min single‑shot gradient (PepSep 25 cm column, nanoElute®, TIMScore™ spectral library).
• Targeted example: 1,565 precursors (Heavy/Light) from 565 proteins in a prm‑PASEF® experiment (Biognosys PQ500 kit example).

Applications
Suitable for high‑throughput proteomics, metabolomics and lipidomics workflows, targeted quantitation, environmental and food screening, and complex‑matrix analyses where mobility‑enhanced selectivity improves detection and quantitation.

• Non‑targeted metabolomics: extensive MS/MS coverage and clean fragment spectra for annotation and library matching.
• Lipidomics: mobility separation resolves isobaric/isomeric lipids and CCS data enhances annotation confidence.
• Targeted proteomics and ultrahigh‑throughput screening with robust quantitation in complex matrices.

Technical specifications

• Technology: Dual‑TIMS with PASEF® (parallel accumulation serial fragmentation).
• Sequencing speed: >120 Hz (PASEF® mode).
• Duty cycle: near 100% achievable with Dual‑TIMS.
• CCS: collisional cross section measurement supported.
• Ion capacity: ~3× increase with Dual‑TIMS analyzer.
• Ion optics: optimized SRIG configuration for enhanced transfer and sensitivity.
• Acquisition modes: dia‑PASEF®, prm‑PASEF®, high‑throughput targeted workflows.
• Demonstrated performance: ~9,000 protein groups from 200 ng K562 lysate in 35 min (example workflow).