Mass spectrometer ion source APCI II

Atmospheric pressure chemical ionization (APCI) is used for less polar molecules where ESI fails to deliver reasonable quantities of ions. In contrast to ESI, it produces mainly singly-charged ions even for larger molecules. APCI is only limited in the case of thermally unstable compounds, since evaporation of the solvent is done by heating the sample in the nebulizer needle. APCI is often used for metabolomics as well as for drug or pesticide screening. The new Bruker APCI II source is of course compatible to Bruker's unique DirectProbe option DIP. The direct injection probe is a novel smart solid sample introduction technique allowing a rapid analysis of solids. Sample preparation is as simple as dipping a disposable glass capillary into the solid sample. Technical Details Unambiguos identification synthetic compound This example shows the unambiguos identification of a synthetic compound using the autoMSn capability of an amaZon SL ion trap system. During the vaporization of solid (refer to TIC insert) data-dependent MS2 and MS3 spectra are taken. Note that after a simple neutral loss in MS2, MS3 provides rich structural information. Samples were kindly provided by SiChem GmbH, Bremen, Germany.