Photon counting spectrofluorometer FE30

time-integrationfor waterfluorescence lifetime measurement

The FE30 Analytical Fluorescence Spectrometer is a high sensitivity, user-friendly instrument designed for both routine laboratory work and advanced research applications. It is essential for various qualitative and quantitative analyses, offering optimized optical and electronic systems to achieve industry-leading sensitivity. The FE30 delivers a water Raman Signal-to-Noise level up to 15,000:1 (RMS-BG), enabling the measurement of samples with very low emission or concentration. Its extended wavelength range up to 900 nm allows for the detection of long-wavelength emissions, such as those from iodocyanine green. The instrument supports rapid 3D scanning, providing comprehensive spectral information about unknown samples and enabling a full 3D scan in just two minutes, significantly reducing analysis time compared to traditional methods.

Key Features

• Excellent signal-to-noise ratio: 15,000:1 (RMS-BG)
• Fast spectrum scan: speed up to 60,000 nm/min
• Extended wavelength range up to 900 nm
• Spectracle: easy-to-use and powerful software
• Validation program: automatically checks instrument health status

Software

• Spectracle software is designed for ease of use and powerful functionality, utilizing a Workspace concept for efficient creation, calculation, and combination of scans for analysis and reporting.
• Basic functions include Excitation and Emission scans, 3D Scans, Time Scan, Synchronous Scan, Fluorescence, Quantitation, and Validation.
• Users can store routine applications and generate reports with a single click.

Measurement Examples

• Life science: Polarization of Rhodamine 6G
• Food science: Determination of Vitamin B2
• Environmental: 3D spectrum of CDOM in marine water
• Pharmaceutical: Quantitation of Quinine
• Material science: Study of quenching fluorescence
• Industrial: Measurement of brightener

Options and Upgrades

• Thermostated Cell Holder with Circulating Bath: Controls sample cell temperature up to 80°C for biological samples
• Polariser: For protein, enzymology, and cellular studies
• Trace Solid Sample Holder - Adjustable Angle: For powder and thin film samples, adjustable 0–90° horizontally
• Solid Sample Holder - Adjustable Angle: For powder and thin film samples, adjustable 0–90° horizontally
• Solid Sample Holder - High Temperature: For thermo-furo experiments, temperature range 25–300°C
• Water Bath Magnetic Stirring Cell Holder: Adjustable stirring speed, temperature control range ambient–80°C, stirrer size 3x6 mm or 2x7 mm

Characteristics / Technical Specifications

• Excitation source: High Intensity 150 W Continuous Xenon Lamp
• Wavelength Measurement Range: Excitation (EX) 200–900 nm, Emission (EM) 200–900 nm
• Spectral Bandwidth: Excitation and Emission: 1, 2.5, 5, 10, 20 nm (5 steps)
• Wavelength accuracy: Excitation ±2 nm, Emission ±2 nm
• Wavelength repeatability: Excitation ±1 nm, Emission ±1 nm
• Sensitivity: Signal-to-Noise level using Raman peak of water, excitation 350 nm is 750:1 (RMS noise at Raman peak), 15,000:1 (RMS noise at background)
• Optical platform: One-piece cast aluminium base for stability and collimation
• Wavelength scan speed: Max 60,000 nm/min; selectable from 30, 60, 240, 1,200, 2,400, 12,000, 60,000 nm/min
• Scan Interval: 0.1–10.0 nm
• Auto gain: 0–1,000 V adjustable
• Fluorescence Intensity Drift: <1.5% (Ex: 350 nm, Em: 450 nm within 10 min)
• Fluorescence Value Range: 0 to 50,000
• Detector: Photomultiplier tube R928
• Software: Spectracle PC-controlled software
• Sample Chamber Dimension: 247 (W) × 215 (D) × 172 (H) mm
• Instrument Dimension (L×D×H): 660 (W) × 600 (D) × 250 (H) mm
• Weight: 50 kg