The ORCA®-Flash4.0 LT3 is a new scientific CMOS camera for fluorescence imaging, which has been improved from the ORCA-Flash 4.0 released in 2011.
It is equipped with a high level of performance required for fluorescence imaging such as life science applications, and can be used not only for basic research applications but also for integration into various types of equipment.
ORCA is a registered trademark of Hamamatsu Photonics K.K. (China, EU, France, Germany, Japan, UK, USA).
Features
Low readout noise (Standard scan): 1.5 electrons rms, 0.9 electrons median
High speed readout (Rapid rolling, Full resolution): 40 frames/s
Large field of view: 13.312 mm (H)×13.312 mm (V)
High resolution: 2048 (H)×2048 (V)
High quantum efficiency: 82 % (Peak QE)
Applications
Time-lapse imaging
Multidimensional imaging
Ca2+ imaging
DNA chip reading
High S/N eq
High S/N equivalent or better than BSI camera
The graph below compares the readout noise distribution with the Gen II BSI camera* and shows that the ORCA-Flash4.0 LT3 has more pixels with smaller readout noise.
With a low readout noise of 1.5 electrons rms, the ORCA-Flash4.0 LT3 delivers high S/N comparable to or better than a BSI camera, especially in the low light range.
This performance can be demonstrated in high quality multidimensional imaging, as typified by long time-lapse imaging with low excitation light with low phototoxicity and photobleaching.
High speed readout
The ORCA-Flash4.0 LT3 is capable of 40 frames/second readout, which is even faster than the conventional ORCA-Flash4.0 LT+ (30 frames/second). This is useful for applications which requires real time measurement with high temporal resolution.