OverviewThe UV-1900i Plus is a double-beam UV-Vis spectrophotometer using Shimadzu's LO-RAY-LIGHT™ diffraction grating. It delivers 1 nm resolution, low stray light and noise, and ultra-fast scanning up to 29,000 nm/min (190–1100 nm). The instrument supports photometric, spectrum, quantitation, kinetics, time-course and bio-methods for accurate quantitative analysis and detection of low-concentration components. It meets JP/USP/EP requirements for routine validation. Operation is possible from the color touch panel (standalone) or from a PC with LabSolutions UV-Vis; improved CPU performance enhances response speed and data handling.
Key features- Ultra-fast scan: up to 29,000 nm/min (190–1100 nm)
- Low stray light (≤0.5% at 198 nm) and low noise for accurate high‑ and low‑concentration measurements
- Standalone operation via color touchscreen or PC control with LabSolutions
- Multi-language interface with large icons and simplified workflow
Measurement modes- Photometric — single or multi-wavelength photometric measurements (up to eight wavelengths)
- Spectrum — obtain sample spectra by wavelength scanning
- Quantitation — create calibration curves from standards and calculate unknown concentrations
- Kinetics — monitor absorbance changes over time (kinetics or rate methods)
- Time Course — record photometric value changes at a set wavelength over time
- Biomethod — quantify DNA or protein concentrations
Daily operation functions- Assist function — guided procedures and recommended corrections (baseline, auto zero, cell blank)
- Shutdown function — sleep mode to reduce power use and extend lamp life
- Wakeup function — scheduled wake to eliminate warm-up waiting
- Startup validation — optional automatic checks at power ON (wavelength accuracy, repeatability, degradation, noise, baseline flatness/stability, EP/USP checks)
- Network connectivity — data transfer to PC via network; wireless printing and multi-unit printing supported (router required; some features optional)
- Barcode reader and keyboard entry — sample naming and numeric input via USB devices to reduce input errors
Performance highlightsResolution: 1 nm (Czerny-Turner monochromator).
Ultra-fast scanning: up to 29,000 nm/min (190–1100 nm) for tracking rapid reactions.
Stray light: 0.5% max. at 198 nm.
Photometric repeatability: 0.0002 Abs max. (at 0.5 Abs and 1.0 Abs).
High reproducibility for detection of low-concentration samples and accurate quantitation.
Reproducibility / Blank solution table (273 nm)No. — Absorbance of Blank Solution (273 nm)
1 — -0.00001
2 — 0.00001
3 — -0.00002
4 — 0.00002
5 — 0.00001
6 — -0.00003
7 — 0.00001
8 — -0.00004
9 — 0.00001
10 — 0.00005
Standard Deviation σ — 0.000025
Validation and complianceIncludes UV validation software (limited edition) for JIS/JP checks; optional validation software supports USP and EP. Hardware and check routines support JP, USP, EP. When configured with appropriate LabSolutions options, supports data integrity features for FDA 21 CFR Part 11 and PIC/S GMP (user management, authority levels, audit trails).
Additional notesDisplay languages available: Japanese, English, Chinese, Spanish, Portuguese, German, French, Russian. Standalone touchscreen or PC control. Optional expanded memory may be required for certain network or data-transfer functions.
Specifications- Model: UV-1900i Plus
- Optical system: Double-beam, Czerny-Turner monochromator with LO-RAY-LIGHT™ diffraction grating (Shimadzu)
- Wavelength range: 190–1100 nm
- Resolution: 1 nm
- Ultra-fast scan speed: up to 29,000 nm/min
- Stray light: 0.5% max. (198 nm)
- Photometric repeatability: 0.0002 Abs max. (0.5 Abs and 1.0 Abs)
- Measurement modes: Photometric, Spectrum, Quantitation, Kinetics, Time Course, Biomethod
- Display: Color touch panel (standalone); multi-language interface
- Data control: Standalone or PC control with LabSolutions UV-Vis; supports ER/ES compliance options
- Network: Data transfer to PC via network; wireless printing supported (router required; some functions optional)
- Assist, Shutdown, Wakeup, Startup Validation functions (configurable)
- Barcode reader and keyboard entry (USB)
- Compliance: JP, USP, EP validation functions; support for FDA 21 CFR Part 11 and PIC/S GMP via software configuration