Ultra high-performance liquid chromatograph Nexera X4

UHPLClaboratoryLC/MS

Overview
Built on the proven technologies of the Nexera series, the next-generation Nexera X4 Ultra High Performance Liquid Chromatograph (UHPLC) sets a new standard in analytical workflows. Its low-dispersion XPLC architecture delivers superior sensitivity, ultra-sharp peaks and high-resolution separations while advanced fluid control enables consistent results in high-speed, ultra-high-pressure analyses. Designed for laboratories requiring high throughput and minimal downtime, Nexera X4 supports ultra-fast gradients, very low system volume and reliable operation as a stand-alone LC or an LC/MS front-end.

Key Features

• XPLC — Extreme Performance Liquid Chromatography with optimized low-dispersion architecture to minimize band broadening for improved peak shape and resolution.
• Engineered as an LC/MS front-end with Nexflow Tubing for MS to reduce interface-related peak broadening and preserve chromatographic performance.
• Ferrule-free, tool-free column connections with high-pressure tolerance for simplified setup and robust sealing.
• Novel capillary PDA flow cell offering enhanced PDA sensitivity, improved baseline stability and reduced dispersion.
• Supports analytical miniaturization with excellent performance on small internal diameter columns to reduce solvent consumption.
• High system reliability reduces reruns and troubleshooting, increasing laboratory throughput and operational efficiency.

Application Highlights
Application notes and technical guides support method design, validation and optimization for demanding workflows: peptide mapping retention time repeatability, ultra-fast LC–MS analyses, solvent consumption reduction and achieving sharper peaks with higher sensitivity when using Nexera X4.

Quick Specs (table)
Maximum Operating Pressure : 1300 bar
ECBB (5σ) : 7 µl
Flow rate settings range : 0.0001 to 2.0000 mL/min
Range of gradient mixing ratio : 0 to 100 % (0.1 % step)
Number of selectable solvents : Up to 4 solvents per pump (maximum 2 pumps)
Injection cycle time : ≤ 6.7 seconds (under specified conditions)
Cross-contamination : ≤ 0.0003 % (with rinse, typically)
Sampling rate : 1 to 250 Hz
PDA Linearity : 3.0 AU (under specified conditions, typically)

Technical specifications

• Maximum Operating Pressure: 1300 bar
• Effective Column Band Broadening (ECBB, 5σ): 7 µl
• Flow rate settings range: 0.0001 to 2.0000 mL/min
• Range of gradient mixing ratio: 0 to 100 % (0.1 % step)
• Number of selectable solvents: Up to 4 solvents per pump (maximum 2 pumps)
• Injection cycle time: ≤ 6.7 seconds (under specified conditions)
• Cross-contamination: ≤ 0.0003 % (with rinse, typically)
• Sampling rate: 1 to 250 Hz
• PDA Linearity: 3.0 AU (under specified conditions, typically)
• Designed for LC/MS front-end use with Nexflow Tubing to reduce interface-related dispersion
• Ferrule-free, tool-free column connections with high-pressure tolerance
• Capillary PDA flow cell for improved sensitivity and baseline stability
• Supports analytical miniaturization and reduced solvent consumption