UHPLC chromatograph VQ-METHOD-DEV

HPLClaboratoryfor pharmaceutical applications

Overview
Accelerate and automate HPLC and UHPLC method development with Thermo Scientific Vanquish Method Development systems. These modular systems enable automated column and eluent scouting across multiple columns and up to 13 eluents, controlled by Chromeleon CDS with downloadable eWorkflows and secure Data Vault storage. Compatible software options (ChromSwordAuto, ChromSword AutoRobust, Fusion QbD) provide closed-loop optimization and robustness testing for small and large molecules.

Highlights

• Automated column and eluent screening: Screen multiple columns and up to 13 eluents using the solvent selection valve and Viper Method Scouting Kit to automate scouting without user intervention.
• Universal and sensitive detection: Support for Charged Aerosol Detector, DAD/PDA, fluorescence, MWD/VWD, RI and MS (ISQ EC/EM) for identification and impurity profiling.
• Software-assisted method development: Control and automate end-to-end workflows via Chromeleon CDS and compatible method development packages (ChromSwordAuto, Fusion QbD).
• Secure centralized data: Chromeleon Data Vault secures backup and centralized access to method and result data for compliance.
• Biocompatible configurations: Vanquish Flex and Vanquish Horizon configurations support high-salt gradients and large-biomolecule separations with dedicated algorithms for biomolecules.
• Automated validation workflows: ChromSword AutoRobust and Chromeleon templates accelerate robustness testing and method validation.

Stages of HPLC method development

• Method scouting: Automated screening of column and mobile phase combinations (Viper fittings, solvent kits, eWorkflows).
• Method optimization: Iterative optimization of gradient, buffer and column selection supported by ChromSwordAuto and Fusion QbD.
• Robustness testing: DOE-based variability assessment automated with ChromSword AutoRobust or Fusion QbD.
• Method validation: Documentation, secure storage and standardized templates via Chromeleon Data Vault and Chromeleon Extension Pack.

Additional product features

• Solvent selection valve and Viper Method Scouting Kit for automated screening workflows.
• Wide detector compatibility for comprehensive detection and identification.
• Chromeleon CDS integration with downloadable eWorkflows and secure Data Vault backup.
• Modular design configurable for UHPLC or HPLC (Vanquish Horizon, Vanquish Flex, Vanquish Core components).
• SmartInject technology for improved injection precision and column lifetime.
• Optional touchscreen and solvent monitoring depending on configuration.

System specifications (overview)
Category: Vanquish Method Development HPLC and UHPLC Systems
System pressure, maximum: 700, 1000 or 1500 bar (configuration dependent)
Flow range: up to 10 mL/min depending on pump selection
Pump options: Binary, Quaternary, Dual, Isocratic (various delay volumes)
Biocompatible: Yes (configuration dependent)
pH range: typically 2–12 (or up to 1–13 depending on chloride concentration and configuration)
System design: Modular
Touchscreen UI compatible (optional): Yes
Solvent monitoring compatible (optional): Yes
Customizable gradient delay volume: Yes
SmartInject: Yes
Injection volume range: standard 0.01–25 µL or 0.01–100 µL (Vanquish Flex/Horizon); optional up to 0.01–1000 µL (Vanquish Core) depending on sampler
Sample cooling: 4–40 °C (sampler dependent)
Sample capacity: standard 4 × 54 vials (≤1.5 mL) plus carousel options for larger vials
Column temperature control: 5–120 °C (selection dependent)
Detector choices: Charged Aerosol Detector; Diode Array (PDA/DAD); Fluorescence; MWD/VWD; RI; Mass Spectrometer

Component summary
Systems are composed of configurable Vanquish Horizon/Flex/Core pumps, multiple autosampler options (including Vanquish Charger for extended capacity), up to three column compartments, Viper Method Scouting Kit and a range of detectors. Detailed specification sheets available from manufacturer resources.

Technical specifications

• Max system pressure: 700 / 1000 / 1500 bar (config dependent)
• Flow range options: 0–5, 0–8 or 0–10 mL/min (pump dependent)
• Pumps: Binary, Quaternary, Dual, Isocratic (various delay volumes)
• pH operating ranges: typically 2–12 (or broader depending on chloride concentration)
• Injection volumes: 0.01 µL up to 1000 µL depending on sampler
• Sample cooling: 4–40 °C (sampler dependent)
• Sample capacity: standard 4 × 54 vials + carousel options
• Column temperature control: 5–120 °C (selection dependent)
• Supported detectors: Charged aerosol, PDA/DAD, fluorescence, MWD, VWD, RI, MS
• Software integration: Chromeleon CDS, ChromSwordAuto, ChromSword AutoRobust, Fusion QbD