Electrophoretic light scattering particle size analyzer Zetasizer Pro

laserdynamic light scatteringoptical

Overview
The Zetasizer Pro is a laboratory analyzer for routine measurement of particle size, molecular size, electrophoretic mobility and zeta potential. It combines DLS, ELS and M3‑PALS measurement modes with NIBS optics and ZS Xplorer software for method setup and data analysis.

Features

• Dynamic Light Scattering (DLS) for size measurement from 0.3 nm to 10 μm.
• M3‑PALS (Mixed‑Mode Phase Analysis Light Scattering) with Constant Current Mode for zeta potential and electrophoretic mobility.
• Electrophoretic Light Scattering (ELS) for zeta potential and stability assessment.
• Non‑Invasive Back Scatter (NIBS) optics for wide dynamic concentration range and high sensitivity.
• Optical filter wheel with fluorescence and polarization filters to measure fluorescent samples and support DDLS.
• Adaptive Correlation to speed up measurements and improve reproducibility with minimal sample preparation.
• Deep learning–based data quality assessment that flags issues and gives improvement guidance.
• Range of disposable and reusable cells; on‑site upgrade path to Zetasizer Ultra; optional MPT‑3 autotitrator for pH studies.

Industry applications

• Academic research: routine characterization of particle/molecular size and zeta potential.
• Bioscience & pharmaceuticals: stability and formulation studies for biological molecules, proteins and viral particles.
• Food & beverage: particle and surface charge analysis to optimise appearance and stability of formulations.
• Nanomaterials, ceramics and pigments: size distribution, dispersion and stability analysis.
• Paints, inks & coatings: formulation dispersibility and finish control.

Key applications

• Proteins: low‑volume measurements using capillary cells.
• Adeno‑associated virus (AAV): MADLS for particle concentration and size distribution.
• Fluorescent nanoparticles: fluorescence filters remove interference for accurate DLS data.
• Ceramics & TiO2: particle populations and stability including salt/pH effects using autotitration.

Compliance and software
OmniTrust supports multi‑instrument regulatory compliance and data integrity (including 21 CFR Part 11). ZS Xplorer software controls the instrument, manages methods and performs advanced data analysis.

Technical specifications (selected)

• Measurement principles: NIBS (Non‑Invasive Back Scatter), DLS, ELS (M3‑PALS).
• Measurement angles: 173°, 13°.
• Size range (DLS): 0.3 nm – 10 μm.
• Minimum sample volume (size): 12 μL.
• Zeta size range: 3.8 nm – 100 μm; minimum zeta volume: 20 μL.
• Concentration capability: examples — Blue Label: 0.2 mg/mL (15 kDa); Red Label: 0.1 mg/mL (15 kDa); maximum up to ~40% w/v (sample dependent).
• Sample conductivity (zeta): up to 260 mS/cm.
• SLS molecular weight range: 300 Da – 20 MDa (±10%).
• Detector: Avalanche Photodiode. Laser: He‑Ne 633 nm (Blue 4 mW / Red 10 mW max).
• Temperature control: 0 °C – 120 °C (dry nitrogen purge required below dewpoint).
• Dimensions (W×D×H): 322 × 565 × 245 mm. Weight: 19 kg. Power: AC 100–240 V, 50–60 Hz.
• Interface: USB 2.0 or higher. Standards: ISO22412:2025, ISO13099‑2:2025, relevant USP and ASTM references.