Two-dimensional liquid chromatograph Sepbox 2D-2000

HPLCfor pharmaceutical applicationslaboratory

Overview
The Sepbox 2D-2000 is an automated two-dimensional purification system that combines advanced preparative HPLC with solid-phase extraction (SPE) to enable fast, automated isolation of natural compounds. Its patented 2D technology is designed for high-throughput workflows, reducing hands-on time while improving reproducibility and overall throughput.

Key features

• Patented two-dimensional technology combining HPLC and SPE
• Detection: UV and ELSD
• Pump / Operation mode: Prep HPLC mode; fully automated operation
• Compact, modular and fully automated system design

Performance and throughput

• Typical isolate capacity: up to 2,000 mg per run
• Able to separate a full extract in a single day
• Automatic collection capacity: up to 576 high-yield fractions per run
• Designed for scalable high-throughput natural product and drug-discovery workflows

Purity & recovery

• Typical recovery: >90% for polar and non-polar compounds
• Purity: up to ~30% of fractions reaching 90–99% purity (measured by ELSD)

Benefits

• Automated operation reduces hands-on time and human error
• High throughput and large isolate capacity accelerate screening and purification campaigns
• Reproducible accuracy for isolation of diverse extract types
• Efficient design lowers operational cost per isolated compound

Applications

• Natural product research
• Compound library isolation
• Drug discovery and lead identification
• Preparative fractionation of complex extracts

Caractéristiques / spécifications techniques

• Model: Sepbox 2D-2000
• Technology: Two-dimensional (HPLC + SPE) patented workflow
• Detection: UV and ELSD
• Pump / Mode: Prep HPLC mode (automated)
• Maximum isolate per run: up to 2,000 mg
• Automatic fraction collection: up to 576 fractions
• Typical recovery: >90%
• Typical fraction purity (ELSD): many fractions 90–99% (up to ~30% of fractions)
• Design: compact, modular, fully automated
• Primary use: high-throughput purification and isolation of natural compounds