Protein sequencer PPSQ series

specimenhigh-speedhigh-accuracy

Overview
The PPSQ is an automated system for determining N-terminal amino acid sequences of proteins and peptides by combining an Edman reaction module with high-performance liquid chromatography (HPLC). Two model variants are available: PPSQ-51A (single reactor) and PPSQ-53A (three reactors). The PPSQ-53A enables continuous sequential analysis of multiple samples.

Benefits of using Edman degradation for amino acid sequencing

• Direct determination of the N-terminal sequence
• No sample pre-treatment required in many cases
• Ability to differentiate isobaric residues (leucine vs isoleucine)
• Can identify proteins not present in sequence databases

Key features (summary)

• Automated Edman degradation with online derivatization to produce stable PTH-amino acids
• Two models: PPSQ-51A (1 reactor) and PPSQ-53A (3 reactors) for single or continuous multi-sample workflows
• Online injection of PTH-amino acids into an HPLC system for separation and detection
• Support for isocratic and gradient HPLC modes; gradient option includes a high-sensitivity flow cell for trace analysis
• LabSolutions PPSQ software for chromatogram processing and amino acid sequence estimation; supports FDA 21 CFR Part 11 features

Features (detailed)

• Edman reaction module: stepwise N-terminal cleavage and derivatization producing PTH-amino acids for reliable identification
• Isocratic system: separates PTH-amino acids isocratically to improve baseline stability and reproducibility for routine analyses
• Gradient system: high-sensitivity flow cell and gradient elution for trace-level PTH-amino acid detection and sequential analysis of low-abundance samples
• Multi-reactor configuration (PPSQ-53A): enables queued, continuous processing of multiple samples to increase throughput
• Data handling: HPLC chromatograms are saved to PC; LabSolutions PPSQ software performs chromatogram processing, peak identification and sequence estimation with audit trail and user management options

Applications (documents table)
Amino Acid Sequence Analysis of Peptides Containing Modified Amino Acids Using The PPSQ-50 Gradient System | 2025-06-10
Amino Acid Sequencing of Synthetic Peptides Using a MALDI-TOF Mass Spectrometer and Protein Sequencer | 2025-04-13
Application of Glatiramer Acetate Analysis with Protein Sequencer PPSQ-50A | 2024-09-10

Technical Documents (table)
Guide to Biopharmaceutical Solutions | 2021-09-01

Manuals (table)
Instruction Manual | 2024-02-05

Downloads (available brochures)
PPSQ-51A/53A Isocratic System brochure (product PDF)
PPSQ-51A/53A Gradient System brochure (product PDF)
Membrane Holder Kit — sample preparation kit documentation
Instruction Manual (product PDF)

Technical specifications

• Model variants: PPSQ-51A (1 reactor) and PPSQ-53A (3 reactors)
• Sequencing chemistry: Edman degradation with online derivatization yielding PTH-amino acids
• Separation/detection: HPLC with support for isocratic and gradient modes; high-sensitivity flow cell available for trace analysis
• Software: LabSolutions PPSQ for chromatogram processing and amino acid sequence estimation; supports FDA 21 CFR Part 11 features (user management, audit trail, security)
• Continuous operation: PPSQ-53A supports continuous sequential analysis of multiple samples
• Analytical advantages: guaranteed N-terminal sequencing, minimal sample preparation, differentiation of isobaric residues, identification of unknown proteins