SEM microscope Volumescope 2

for materials research3Din-situ

3D SEM for large volume samples with serial block face imaging and multi energy deconvolution. Volumescope 2 Scanning Electron Microscope for serial block face imaging The Thermo Scientific Volumescope 2 Scanning Electron Microscope (SEM) is our state-of-the-art serial block-face imaging system. Keep control of experiments with easy to use technology and protect a wide range of possible samples with tried and tested solutions for every acquisition step. Block face imaging Unraveling the complex 3D architecture of cells and tissues in their natural context is crucial for structure-function correlation in biological systems and soft materials. Serial block-face SEM (SBF-SEM) combines in situ sectioning and imaging of plastic-embedded tissue blocks within the SEM vacuum chamber for automated, 3D reconstruction of large tissue volumes. Axial resolution was once limited by the minimum section thickness, but truly isotropic resolution is now possible with the addition of multi-energy deconvolution. 3D SEM Following in situ sectioning of the block-face using a diamond knife, the freshly exposed tissue is imaged several times using increasing accelerating voltages. These images are subsequently used in a deconvolution algorithm to derive several optical subsurface layers, forming a 3D subset. By repeating this cycle, the Volumescope 2 SEM offers isotropic datasets with 10 nm Z-resolution. Polymer microstructures High-resolution imaging in 3D is of great importance when trying to understand the microstructure and properties of polymer materials.