Laboratory workstation AI-5000E

automaticanaerobic

Product overview
AI-5000E anaerobic and hypoxic workstation for inoculation, continuous cultivation, passage, medium exchange, observation and identification of microorganisms under anaerobic and low-oxygen conditions. Supports cultivation of anaerobic and microaerophilic bacteria and cell culture experiments with precise O2 and CO2 control; supports intermittent hypoxia, variable oxygen profiles and extreme hypoxia experiments.

Operating conditions
Power supply: 220 V, 50/60 Hz
Max current: 4 A
Environmental condition: ambient laboratory environment
Temperature reference: 25°C; relative humidity <90%

Functional features

• Precise oxygen control to simulate in vivo low-oxygen environments.
• CO2 control for optimal cell culture conditions.
• Fully automatic ultrasonic humidification and humidity control.
• Built-in HEPA U16 filtration system and UV sterilization lamp.
• Integrated long-sleeve manipulation system for sealed handling without external vacuum pump.

Technical parameter of Anaerobic Hypoxic Workstation

• Built-in fast transfer box (12 L) for multiple 90 mm plates; N2 blow transfer completes within 60 seconds.
• Intelligent transfer gate protection to preserve internal atmosphere.
• One-button, one-hand operation for transfer gate.
• Large internal capacity: up to 760×90 mm plates; >80×250 mL triangular bottles; >50×500 mL triangular bottles; >100×250 mL reagent bottles; >80×500 mL reagent bottles; >100 homogeneous bags.
• Removable side panel for access and cleaning.
• Anaerobic mode: dual gas (anaerobic mix 80% N2 + 10% CO2 + 10% H2 plus N2); low-oxygen mode: CO2, N2 and compressed air.
• 7-inch color touchscreen for parameter setting, monitoring, alarms and USB data export.
• Ultrasonic humidification with automatic control; humidity adjustable down to -90% of ambient with automatic low-water stop.
• Fluorescent lamp and 254 nm UV sterilization lamp included.
• Built-in HEPA (U16) filter, efficiency 99.9995% @ 0.12 μm; meets ISO14644-1 Class 3.
• Long-sleeve system with fully sealed design to prevent introduction of 100% oxygen; no external vacuum pump required.
• 6-stage O2/CO2 gas circulation with programmable targets, time and cycles; experiment profiles and USB data download supported.

Key parameters
Oxygen control range: anaerobic mode <5 ppm; hypoxic mode 0–25% adjustable by 0.1%.
CO2 control range: 0–20% adjustable by 0.1%; O2 and CO2 sensors with calibration mode (no manual calibration required).
Temperature control: room temperature +3°C to 52°C; display accuracy 0.1°C; average deviation ±0.3°C; temperature test report available.
Power sockets: 2 built-in waterproof sockets.
Data recording: USB data recorder logging O2, CO2, temperature, humidity and intake time; recording up to 30 days.
Alarms: high/low temperature, humidity range exceed, low pressure for anaerobic mix, N2, CO2, compressed air; gas leak alarm; automatic gas overcurrent protection closing intake solenoid after >5 minutes continuous intake.
Timed gassing: configurable gassing time displayed on touchscreen; fast mode time freely set and displayed.
Palladium catalyst: efficient palladium catalyst included; long life up to 4 years without frequent re-activation.
Equipment dimensions: 1285 × 735 × 685 mm (L × H × D).

Main configuration

• Host: 1 set
• Long sleeves: 1 pair
• HEPA filtration system: 1 set
• Oxygen sensor: 1 set
• Carbon dioxide sensor: 1 set
• Multi-channel gas mixer: 1 set
• Gas gradient circulation system: 1 set
• Ultrasonic humidification system: 1 set
• Dish racks: 8 sets
• LED lamp: 1 set
• UV lamp: 1 set
• Catalyst: 3 bags (200 g)
• Detoxification: 3 bags (200 g)
• Lubrication powder (sleeve): 1 bottle
• Internal power socket: 2 sets
• Power cord: 1 set
• Operation manual: 1 set
• Installation tools: 1 set

Characteristics / Technical specifications

• Model: AI-5000E workstation for anaerobic and hypoxic microbial and cell culture.
• Gas control: multi-gas control (O2, CO2, N2, H2, compressed air) with programmable cycling and 6-stage control for experiment simulation.
• Environmental control: temperature, humidity, O2 and CO2 tightly regulated with touchscreen control and data logging.
• Filtration & sterility: built-in HEPA U16 filter and UV sterilization; palladium catalyst for oxygen removal.
• Transfer system: built-in fast transfer box (12 L) with N2 transfer in ~60 seconds and intelligent transfer gate protection.
• User interface: 7-inch color touchscreen for settings, monitoring, alarms and USB export.
• Dimensions and capacity: 1285×735×685 mm; large internal capacity supporting plates, bottles and bags as specified.