Ultra high-performance liquid chromatograph Nexera XS inert

laboratoryfor pharmaceutical applicationsfor impurities analysis

Overview
The Nexera XS inert UHPLC system addresses analyte adsorption and corrosion challenges in biomolecule analysis by combining UHPLC pressure capability with a fully bioinert sample flow path (no wetted metal surfaces). This design improves recovery and sensitivity for metal‑adsorbing compounds while maintaining compatibility with high‑salt and extreme pH mobile phases.

Key features

• Bioinert sample flow path: elimination of wetted metal surfaces to reduce adsorption of proteins, mAbs, oligonucleotides and other metal‑sensitive analytes.
• Elevated pressure tolerance: UHPLC performance, configurable up to 1050 bar for small‑particle columns.
• High corrosion resistance: suitable for high‑salt and extreme‑pH mobile phases.
• Improved recovery and sensitivity: reduced sample loss due to adsorption, better peak area reproducibility.
• Enhanced peak shape and resolution: optimized for bioseparation methods.
• Method support and Analytical Intelligence: features for method development, method scouting and routine QC.

Applications

• Separation and analysis of biomolecules prone to metal adsorption: proteins, monoclonal antibodies, oligonucleotides, ADCs.
• Chromatographic modes: SEC, IEX, HIC, reversed‑phase ion‑pair for oligonucleotides, and other bioseparation techniques.
• Use cases: bioanalytical method development, method scouting, QC and LC‑MS workflows.

Videos

• Nexera XS inert — Overview
• Nexera XS inert UHPLC: Clearly Higher Peaks for Bioanalysis
• New Breakthrough in Biologics Analysis with Bioinert, Biocompatible and High-Pressure UHPLC

Downloads

• Oligonucleotide Therapeutics Solution Guide [PDF]
• Shim-pack HPLC Columns for Bioseparation [PDF]
• Nexera XS inert brochure [PDF]

Applications & technical documents (selected)

• Exploration and Optimization of Efficient Separation Conditions for Oligonucleotides by Reversed‑Phase Ion‑Pair Chromatography
• Improvement of Oligonucleotide Peak Shape Using Automatic Pretreatment Function (Co‑injection)
• Analysis of Oligonucleotide Impurities Using Single Quadrupole Mass Spectrometer
• Improvement of Data Reliability by Real‑Time Measurement of Eluent pH
• Method Optimization for the Analysis of Monoclonal Antibodies by Size‑Exclusion Chromatography

Technical specifications

• System type: UHPLC with bioinert sample flow path (no wetted metal surfaces).
• Flow path: metal‑free wetted surfaces (PEEK and other inert materials).
• Max. pressure: up to 1050 bar (configuration dependent).
• Pump/gradient: parallel dual‑piston pump for binary/quaternary gradients, flow rates up to 10 mL/min (config dependent).
• Injection: Needle‑in‑Flow direct injection, injection range ~0.1–50 µL.
• Detection options: UV, PDA/DAD with inert flow cells, fluorescence, RI, ELSD, CDD, single‑quadrupole LC‑MS.
• Compatible methods: SEC, IEX, HIC, RP ion‑pair and other bioseparation techniques.
• Materials & benefits: bioinert, corrosion‑resistant design to reduce adsorption and improve data reliability for bioanalysis.