Overview Densitometer DEN-1B measures turbidity of cell suspensions in the standard range 0.0–6.0 McFarland units (approx. 0 – 180×10^7 cells/ml) with digital output in McFarland units. Extended measurement up to 15.00 McF is possible, noting increased standard deviation at higher values. The unit is factory calibrated for 16 mm glass tubes and retains calibration without power. Multi-point calibration (2–6 points) within 0.0–6.0 McF is supported. Use of Biosan calibration standards is recommended for full traceability; commercial or laboratory-prepared standards (e.g., BaSO4) are acceptable. Factory calibration can be restored if required.
Applications Measurement of microbial cell concentration (bacteria, yeast) during fermentation and culture growth; determination of microorganism sensitivity to antibiotics; identification with test systems; measurement of absorbance at a fixed wavelength; quantitative estimation of coloured solutions that absorb green light.
Calibration kits available - CKG16 — for glass tubes Ø16 mm: set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland turbidity standards (latex particles). Cat. No.: BS-050102-BK
- Calibration kit for glass tubes Ø18 mm — set of 0.5; 1.0; 2.0; 3.0; 4.0; 5.0 McFarland standards (BaSO4). Cat. No.: 70900
- Calibration kit for glass tubes Ø12 mm — set of 0.0 (blank); 0.5; 2.0; 3.0 McFarland standards (latex particles). Cat. No.: 21255
Versions - DEN-1 — powered from external supply
- DEN-1B — powered from external supply and by 3 × AA batteries
Notes Compatible with glass and transparent plastic tubes with external diameters 12 mm, 16 mm (using adapter A-12 or A-16) or 18 mm (no adapter). Adapter A-16 is included; A-12 is optional. Recalibration is required whenever tube diameter, bottom shape or material changes.
Technical specifications - Measurement range: 0.00–15.00 McF
- Resolution: 0.01 McF
- Light source: LED
- Measurement wavelength (λ): 565 ±15 nm
- Accuracy (0.0–6.0 McF): ±3%
- Measurement time: 1 s
- Minimum sample volume: 2 ml
- Tube external diameter: 12 mm, 16 mm (with A-12/A-16 adapter) or 18 mm
- Factory calibration restore: yes
- Display: LCD
- Independent power supply: 3 × AA batteries
- Overall dimensions (W×D×H): 165 × 115 × 75 mm
- Weight: 0.7 kg
- Input current / power consumption: 12 V, 12 mA / 0.15 W
- External power supply: AC 100–240 V, 50/60 Hz → DC 12 V
- Standard set: external power supply, A-16 adapter