OverviewLiquid nitrogen and cryogen free controlled rate freeze and thaw system for cryopreservation - CRFT. The CRFT delivers accurate, precise and reproducible cryopreservation profiles suitable for research and clinical workflows. It supports a wide range of biological material including embryos, stem cells, mammalian cells, spermatozoa, antibodies, tissue sections and rodent organs. Freezing and thawing rates are precisely controlled, with special control over the nucleation/seeding phase to maximise post-thaw recovery. Operation is straightforward and can be performed with or without a PC.
Applications- Transgenic embryology research
- Stem cell research and storage
- Clinical and research samples (e.g. lymphocytes and tissue cell lines in standard cryovials)
- Various mammalian cell types including cardiomyocytes, adipose, liver and muscle cells
- Cord blood derived stem cells
- Adherent cells and stem cells in microplates
- Cell suspensions in numbered or barcoded arrays
- Integration into automated systems
- Suitable for veterinary IVF applications
Key features and benefits- Accurate, reproducible control of cooling and sample temperatures
- Designed to maximise cell survival rates after thawing
- Configurable freezing and thawing protocols (linear and non-linear profiles)
- Liquid nitrogen, alcohol and cryogen free — no topping up required
- Interchangeable heads for workflow flexibility (ordered separately)
- Free software for designing, tracking, exporting and reporting temperature profiles
- Easy to use; samples can be nucleated/seeded in situ
- Low running costs — estimated at ~1% of liquid-nitrogen controlled rate freezing
- Operates from a 24V supply (CRFT EU variant: 100–240VAC 50/60Hz, Euro plug)
- Temperature range: -100°C to 30°C* (minimum head temperature achieved with sealed lid; sample temperatures may vary)
- Servicing and calibration options available; command protocols provided
Characteristics / Technical specifications- Model: CRFT
- Temperature range: -100°C to 30°C (CRFT head; sample temperatures may vary)
- Minimum head temperature achieved with sealed lid in place
- Power / Supply: runs on 24V supply; CRFT EU variant: 100–240VAC 50/60Hz, Euro plug
- Cryogen-free operation: liquid nitrogen, alcohol and cryogen free (no topping up required)
- Interchangeable heads available (ordered separately)
- Software: free application for design, tracking, export and reporting of temperature profiles
- Operation: can be used with or without a PC; in-situ nucleation/ seeding supported
- Low running costs: estimated at ~1% compared with liquid nitrogen controlled rate freezing
- Applications supported: cryovials, straws, bags, microplates and Matrix-96-well block plates
- Distribution: Biosan is the exclusive distributor of CRFT in NIS, Baltic and Eastern European countries