UV-Vis spectrophotometer SP-UM300

LEDxenonabsorption

Description

• User-friendly software, easy to use. Free software updates are available.
• Micro-volumes measuring: only requires 0.5~2μl sample to accurately determine nucleic acids and proteins.
• Fast and easy measurements: turn on and instantly measure without lamp warm-up time; easy measurement within 8 sec.
• Long-life Xenon flash lamp: Xenon flash lamp, 10 flashes, up to 10 years; no dilutions and no expensive consumables.
• No consumables required: direct microvolume measurements from 1–2μL of sample, eliminating the need for expensive accessories.
• No computer required for selected models: touch screen operation on SP-UM300, SP-UM400A, SP-UM500; Android system operation available.
• Full-range wavelength detection for SP-UM100 / SP-UM300 / SP-UM500: 200-800 nm. SP-UM400A is a basic UV micro-volume spectrophotometer designed for 260 nm and 280 nm measurements for nucleic acids and proteins.
• SP-UM500 adds fluorescence detection for quantifying extremely low-concentration nucleic acids with a lower limit of 0.5 pg/μl (using fluorometric mode).

SP-UM100 Highlights

• Turn on and instantly measure without lamp warm-up time; full scan capability from 200-800 nm within 5 sec.
• Detects nucleic acids up to 4500 ng/μl (dsDNA).
• 3864-element linear silicon CCD array.
• Requires connection to a computer; software compatibility WinXP, Win7, Win8.

SP-UM300 Highlights

• Improved micro-spectrophotometer based on SP-UM100 with 200-800 nm range; added OD600 function for bacterial cell concentration detection.
• Android system, 7-inch touch screen with APP software and simple interface; HD touch screen, no PC required.
• 2048-element linear silicon CCD array detector.
• Turn on and instantly measure; full scan 200-800 nm within 6 sec.
• Convenient OD600 function for bacteria/microorganism concentration detection.
• Built-in printer and USB output for data analysis and storage; auto power-off after 5 minutes idle, wake by touching screen.

SP-UM400A Highlights

• Basic micro-volume UV spectrophotometer with three wavelengths (260 nm, 280 nm and 365 nm) for nucleic acids and proteins.
• Android system operation, 7-inch touch screen, APP software, no computer required.
• OD600 function for bacteria/microorganism concentration detection.
• Measures nucleic acid concentration at 260 nm and purity via 260/280 ratio; measures purified protein concentration at 280 nm.
• Data printable via built-in printer; data export via SD-RAM card and USB memory.

SP-UM500 Highlights

• Built on SP-UM300 with added 0.05 mm optical path length and maximum detection concentration up to 15000 ng/μl.
• Added fluorescence detection function for accurate quantification of extremely low-concentration nucleic acids, lower limit 0.5 pg/μl.
• Automatic detection after lowering the arm.
• Patented lifting motor structure to gently stretch liquid column, reducing breakage and improving stability for high-concentration samples; suitable for precise protein quantification.
• Android system, 7-inch capacitive touch screen, intuitive APP software.
• 2048-element linear CCD array detector, detection and display in 6 s; built-in printer or USB export; intelligent light-source management to extend component life.

Fluorescence mode
Fluorescence detection combined with fluorescence quantitative analysis kits enables accurate quantification of DNA, RNA and protein concentrations through specific binding of fluorochrome with the target material; minimum limit reported 0.5 pg/μl (dsDNA). SP-UM500 is compatible with common fluorescence quantitative reagents to maximize convenience and minimize detection cost.

Fluorescence channels (table)
Code | Light | Excitation Filters | Emission Filters
SP-UM500U (optional) | UV | 365±20 nm | 420-480 nm (60 nm)
SP-UM500 (standard) | Blue | 460±20 nm | 525-570 nm (45 nm)
SP-UM500G (optional) | Green | 525±20 nm | 575-640 nm (65 nm)
SP-UM500R (optional) | Red | 625±20 nm | 670-725 nm (55 nm)

Fluorescence channel applications (table)
Channel | Excitation Filters | Normal kits | Applications
UV channel | 365±20 nm | Hoechst 33258, 4-MU, EnZCheK, Caspase | Nucleic acid quantification, plant GUS reporter gene detection, apoptosis detection
Blue channel | 460±20 nm | PicoGreen®, OliGreen, RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™ | dsDNA, ssDNA, RNA quantification, GFP gene detection, fluorescein detection, protein detection
Green channel | 525±20 nm | Rhodamine, Cy3, RFP, Vybrant Cytotoxicity | Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, cell activity/position detection
Red channel | 625±20 nm | Cy5, Quant-iT RNA | Cy-5 fluorescence labeling detection, RNA quantification

General specifications (table)
Type | SP-UM300
Wavelength Range | 200-800 nm
Minimum sample size | 0.5-2.0 μl
Path length | 0.2 mm / 1.0 mm
Light source | Xenon flash lamp (for SP-UM300 family); LED noted elsewhere for some fluorescence modules
Detector type | 2048-linear CCD array (SP-UM300)
Wavelength accuracy | 1 nm
Spectral resolution | ≤3 nm (FWHM at Hg 546 nm)
Absorbance precision | 0.003 Abs
Absorbance accuracy | 1% (7.332 Abs at 260 nm)
Absorbance range | 0.04-90 A
Detects nucleic acid up to | 2-4500 ng/μl (dsDNA)
Measurement time | <5 s (full) / 3 s (once) depending on mode
Data output | USB; built-in printer on selected models; SD/USB storage on SP-UM400A
Operating voltage | 24 VDC
Operating power | 25 W
Standby power | 5 W
Dimension (WxDxH) mm | 210 × 268 × 181 mm
Weight | 2.8 kg
Software compatibility | Android system for SP-UM300/SP-UM400A/SP-UM500; SP-UM100 requires PC (WinXP/7/8)

Fluorescence detection features
Fluorescence detection allows precise quantification of DNA, RNA and protein concentrations using high-sensitivity fluorescence analysis kits. Fluorochromes bind specifically to target molecules; upon excitation, emitted fluorescence intensity is measured and target content is determined against a standard curve. Compared to SP-UM300, the added fluorescence function increases detection sensitivity and lowers the detection limit; fluorescence detection maintains strong specificity even when RNA is present during DNA detection.

Technical specifications

• Model: SP-UM300 (SKU: SP-UM300)
• Wavelength range: 200-800 nm
• Minimum sample size: 0.5–2.0 μl
• Path length options: 0.2 mm and 1.0 mm (SP-UM300); SP-UM500 offers additional 0.05 mm optical path option
• Light source: Xenon flash lamp (long-life xenon flash for microvolume units); LED noted for fluorescence modules
• Detector: 2048-element linear CCD array (SP-UM300); other models vary (e.g., SP-UM100 3864-element CCD)
• Wavelength accuracy: 1 nm
• Spectral resolution: ≤3 nm (FWHM at Hg 546 nm)
• Absorbance precision: 0.003 Abs
• Absorbance accuracy: ~1% at 260 nm (example given 7.332 Abs)
• Absorbance range: 0.04–90 A
• Nucleic acid detection range: ~2–4500 ng/μl (dsDNA) depending on model and mode
• Fluorometric lower detection limit (SP-UM500): down to 0.5 pg/μl (with appropriate kits)
• Measurement speed: full scan 200–800 nm in 5–6 s depending on model; single measurement ~3 s
• Data output: USB; built-in printer available on selected models; SD/USB export on SP-UM400A
• Power: 24 VDC, operating 25 W, standby 5 W
• Dimensions (WxDxH): 210 × 268 × 181 mm; Weight: 2.8 kg
• Interface/OS: Android system on SP-UM300/SP-UM400A/SP-UM500; SP-UM100 requires PC (WinXP/7/8)
• Special functions: OD600 for bacterial concentration; automatic detection on arm drop (SP-UM500); patented lifting motor to improve liquid column stability (SP-UM500)