UV-Vis spectrophotometer SP-UM100

LEDxenonabsorption

Description

• User-friendly software with free updates; touch-screen operation available on selected models.
• Microvolume measurement: accurate quantification from 0.5–2 μl for nucleic acids and proteins without consumables.
• Instant measurement with xenon flash lamp—no warm-up, fast scan times (≈5–8 s according to model).
• Fluorometric mode (SP-UM500) for low-concentration samples; fluorometric detection down to 0.5 pg/μl with compatible assays.
• Models available with full-range detection (200–800 nm) or dedicated wavelengths for nucleic acids/proteins.

SP-UM100 Highlights

• Full-range UV-Vis microvolume spectrophotometer (200–800 nm) with instant measurement; full scan in <5 s.
• Detects dsDNA up to ~4500 ng/μl; minimum sample 0.5–2 μl.
• 3864-element linear silicon CCD array detector.
• PC connection required for data output; compatible with legacy Windows versions.

SP-UM300 Highlights

• Enhanced micro-spectrophotometer based on SP-UM100 with added OD600 function for cell density measurement.
• Android OS with 7" capacitive touch screen and APP interface—standalone operation (no PC required).
• 2048-element linear silicon CCD array; full-range scan (200–800 nm) ~6 s.
• Built-in printer and USB data export; automatic standby and touch wake.

SP-UM400A Highlights

• Basic microvolume UV spectrophotometer optimized for nucleic acids and proteins (three wavelengths: 260, 280 and 365 nm).
• Android 7" touch operation; no PC required; APP software for routine workflows.
• Includes OD600 for microorganism concentration measurements; built-in printing and SD/USB export.

SP-UM500 Highlights

• Advanced model with optional 0.05 mm path length and highest measurable concentration up to 15000 ng/μl.
• Integrated fluorescence detection for trace quantification (dsDNA down to 0.5 pg/μl with suitable reagents).
• Patented lifting motor for sample protection and stable measurements with high-concentration samples.
• Android 7" touch interface, 2048-element CCD, built-in printer and USB export; full-range detection/display in ~6 s.

Fluorescence mode (overview)
Fluorescence detection combined with compatible quantitation kits enables highly sensitive measurement of DNA, RNA and proteins via specific fluorochrome binding; detection limits depend on assay and reagents used (example: SP-UM500 can reach 0.5 pg/μl dsDNA with appropriate kits).

Fluorescence channels (code | excitation | emission)
SP-UM500U (optional) | 365±20 nm | 420–480 nm (60 nm)
SP-UM500 (standard) | 460±20 nm | 525–570 nm (45 nm)
SP-UM500G (optional) | 525±20 nm | 575–640 nm (65 nm)
SP-UM500R (optional) | 625±20 nm | 670–725 nm (55 nm)

Fluorescence detection specification (selected)

• Light source: LED
• Dynamic range: five orders of magnitude
• Linear dynamic range: R2 > 0.995
• Detector: photodiode
• Repeatability: < 1.5%
• Stability: < 1.5%
• Example sensitivity: dsDNA 0.5 ng/ml
• Measurement speed: 3 s (single)

Applications of fluorescence channels (summary)
UV channel (365±20 nm): Hoechst33258, 4-MU — nucleic acid quantification, reporter assays, apoptosis detection.
Blue channel (460±20 nm): PicoGreen®, OliGreen — dsDNA/ssDNA/RNA quantification, GFP and fluorescein detection.
Green channel (525±20 nm): Rhodamine/Cy3, RFP — fluorescence labeling, RFP detection, cell activity assays.
Red channel (625±20 nm): Cy5, Quant-iT RNA — Cy5 labeling, RNA quantification.

Technical specifications (selected)

• Models: SP-UM100, SP-UM300, SP-UM400A, SP-UM500
• Wavelength range (full models): 200–800 nm
• Minimum sample size: 0.5–2.0 μl
• Path length options: 0.05 mm (option), 0.2 mm, 1.0 mm
• Light source: Xenon flash lamp (microvolume mode), LED for fluorescence
• Detector: 2048- or 3864-element linear CCD array (model dependent); photodiode for fluorescence
• Wavelength accuracy: 1 nm; spectral resolution ≤ 3 nm
• Absorbance precision: 0.003 A; absorbance range: 0.04–90 A
• Concentration range (examples): dsDNA ~0.5 pg/μl (fluorescence) to 15000 ng/μl (optical path option)
• Measurement time: full scan < 5–8 s depending on model
• Power: 24 V DC, typical 20 W; standby 5 W
• Dimensions (W×D×H): 200 × 250 × 166 mm; weight: ~2.6 kg
• Data output: USB, SD, built-in printer or PC connection (model dependent)