Visible spectrophotometer SP-UM400A

UV-VisUVLED

Description

• User-friendly software, easy to use. Free software updates are available.
• Micro-volumes measuring: only requires 0.5–2 μL sample to accurately determine nucleic acids and proteins.
• Fast and easy measurements: turn on and instantly measure without lamp warm up time; easy measurement within 8 s.
• Long life Xenon flash lamp: Xenon flash lamp, 10 flashes, up to 10 years, no dilutions and no expensive consumables.
• No consumables required: direct microvolume measurements from 1–2 μL of sample; eliminates the need for expensive accessories.
• No computer required for some models: touch screen operation available on SP-UM300, SP-UM400A, SP-UM500; Android system operation on SP-UM300, SP-UM400A, SP-UM500.
• SP-UM100 / SP-UM300 / SP-UM500 provide full wavelength detection ability from 200–800 nm; SP-UM400A is a basic UV spectrophotometer specifically designed for nucleic acids and proteins with selected wavelengths.
• Fluorometric mode (on SP-UM500) available for very low concentration samples: for concentrations below 2 ng/μL, fluorometric mode can be used; minimum detection limit can reach 0.5 pg/μL.

SP-UM100 Highlights

• Turn on and instantly measure without lamp warm up time; full scan capability from 200–800 nm within 5 s.
• Detects nucleic acids up to 4500 ng/μL (dsDNA).
• 3864-element linear silicon CCD array detector.
• Requires PC connection; software compatibility: WinXP, Win7, Win8.

SP-UM300 Highlights

• Improved micro-spectrophotometer based on SP-UM100 with 200–800 nm and added OD600 function for bacterial cell concentration detection.
• Android system, 7-inch touch screen, APP software, simple interface; built-in HD touch screen, no PC required.
• 2048-element linear silicon CCD array detector; full scan capability from 200–800 nm within 6 s.
• Data can be printed with built-in printer and output via USB.
• Auto power-off after idle 5 minutes; wake by touch.

SP-UM400A Highlights

• Basic micro-volume UV spectrophotometer designed for measurement of nucleic acids and proteins using three wavelengths: 260 nm, 280 nm and 365 nm.
• Android system operation, 7-inch touch screen, built-in APP software; no computer required.
• Includes OD600 function to detect concentration of bacteria and microorganisms.
• Measures nucleic acid concentration at 260 nm and purity using ratio 260/280; measures purified protein concentration at 280 nm.
• Data can be printed with built-in printer; data output via SD-RAM card and USB memory.

SP-UM500 Highlights

• Based on SP-UM300 with added features: 0.05 mm optical path length option and highest detection concentration up to 15,000 ng/μL.
• Added fluorescence detection function for accurate quantification of extremely low concentration nucleic acids with lower limit of 0.5 pg/μL.
• Automatic detection after putting down the arm; patented lifting motor structure to protect liquid column and improve reading stability at high concentrations.
• Android system, 7-inch capacitive touch screen; 2048-element linear CCD array detector; detection and display in ~6 s.
• Longer service life of optical components with intelligent user identification and auto light-off after 5 minutes idle; results printable via built-in printer or exportable via USB.

Fluorescence channel / filters (SP-UM500 variants)
SP-UM500U (optional) — Light: UV; Excitation filter: 365 ± 20 nm; Emission filter: 420–480 nm (60 nm)
SP-UM500 (standard) — Light: Blue; Excitation filter: 460 ± 20 nm; Emission filter: 525–570 nm (45 nm)
SP-UM500G (optional) — Light: Green; Excitation filter: 525 ± 20 nm; Emission filter: 575–640 nm (65 nm)
SP-UM500R (optional) — Light: Red; Excitation filter: 625 ± 20 nm; Emission filter: 670–725 nm (55 nm)


Fluorescence detection features and applications
The fluorescence detection mode enables precise quantification of DNA, RNA and protein concentrations using fluorescence quantitative analysis kits and specific fluorochromes. Fluorescence detection increases sensitivity and specificity compared to absorbance-only methods and can quantify very low concentration samples when combined with appropriate reagents.

Application of different fluorescence channels (summary)
Channel: UV channel — Excitation: 365 ± 20 nm — Typical kits: Hoechst 33258, 4-MU, EnZCheK, Caspase — Applications: Nucleic acid quantification, plant GUS reporter gene detection, apoptosis detection
Channel: Blue channel — Excitation: 460 ± 20 nm — Typical kits: PicoGreen®, OliGreen, RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™ — Applications: dsDNA, ssDNA, RNA quantification; GFP detection; fluorescein and protein detection
Channel: Green channel — Excitation: 525 ± 20 nm — Typical kits: Rhodamine, Cy3, RFP, Vybrant Cytotoxicity — Applications: Rhodamine detection, Cy3 labeling detection, RFP gene detection, cell activity/location detection
Channel: Red channel — Excitation: 625 ± 20 nm — Typical kits: Cy5, Quant-iT RNA — Applications: Cy5 labeling detection, RNA quantification


Specification summary (selected)
Light source (fluorescence table): LED
Dynamic range (fluorescence table): Five orders of magnitude
Linear dynamic range (fluorescence table): R2 > 0.995
Detector (fluorescence table): Photodiode
Repeatability (fluorescence table): <1.5%
Stability (fluorescence table): <1.5%
Sensitivity (fluorescence table): dsDNA: 0.5 ng/mL
Measurement speed (fluorescence table): 3 s (once)

Ordering information (examples)
XY-11060-00 — SP-UM500 Micro-Spectrophotometer, DC24V 5W (Standard)
XY-11070-00 — SP-UM500U Micro-Spectrophotometer, DC24V 5W (Optional)
XY-11080-00 — SP-UM500G Micro-Spectrophotometer, DC24V 5W (Optional)
XY-11090-00 — SP-UM500R Micro-Spectrophotometer, DC24V 5W (Optional)


Notes
The product page covers a range of micro-volume UV-VIS spectrophotometers (SP-UM100, SP-UM300, SP-UM400A, SP-UM500) with varying features: full-range scanning (200–800 nm) on selected models, touch-screen Android operation on several models, OD600 bacterial detection function on some models, built-in printing and USB/SD output options on multiple models. SP-UM400A is presented as the basic micro-volume UV spectrophotometer focused on nucleic acids and proteins with dedicated wavelengths (260 nm, 280 nm, 365 nm).

Caractéristiques / spécifications techniques

• Model / Type: SP-UM400A
• Wavelength range: 260 nm, 280 nm (SP-UM400A)
• Minimum sample size: 1–2.0 μL
• Path length: 0.5 mm
• Light source: UV LED (SP-UM400A); LED for fluorescence modules
• Detector type: UV-silicon photocell (SP-UM400A)
• Spectral resolution: ≤ 8 nm (FWHM at Hg546 nm)
• Absorbance precision: 0.005 Abs
• Absorbance accuracy: 2% (7.332 Abs at 260 nm)
• Absorbance range: 0.2–50 A
• Detects nucleic acid up to: 10–2500 ng/μL (dsDNA) for SP-UM400A
• Measurement time: < 6 s (SP-UM400A)
• Data output: USB; SP-UM400A can also output via SD-RAM card and USB memory
• Sample pedestal material: Aluminum alloy and quartz
• Operating voltage: 24 V DC
• Operating power: 25 W
• Standby power: 5 W
• Dimension (WxDxH): 210 x 280 x 181 mm
• Weight: 2.0 kg
• Software compatibility / OS: Android system (for SP-UM300, SP-UM400A, SP-UM500); SP-UM100 requires PC (WinXP/Win7/Win8)
• Fluorescent detection (SP-UM500): optional channels with specific excitation/emission filter sets; fluorescence mode min. detection ~0.5 pg/μL (dsDNA) with suitable kits
• SKU on page: SP-UM400A